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adapter sequences for 454 sequencing  (Microsynth ag)

 
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    Microsynth ag adapter sequences for 454 sequencing
    Adapter Sequences For 454 Sequencing, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/adapter+sequences+for+454+sequencing/adapter+sequences+for+454+sequencing/pm25307450-78-26-27
    Average 90 stars, based on 1 article reviews
    adapter sequences for 454 sequencing - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Circulating tumor DNA and circulating tumor cells in metastatic triple negative breast cancer patients.
    Article Snippet: .. One μl of the diluted PCR product was subjected to another round of amplification with 0.4 μM indexing primers containing the adapter sequences needed for 454 sequencing (Microsynth), 0.05 U/μl Fast Start High Fidelity polymerase (Roche) and 200 μM dNTPs in 1X High Fidelity Buffer 2 (Roche) in a reaction volume of 15 μl at 95°C for 3 min, 25 cycles of 95°C for 15 s, 60°C for 45 s, and 72°C for 1 min, followed by 72°C for 8 min. .. The PCR products were analyzed on DNA 1000 chips (Agilent) on the Agilent 2100 Bioanalyzer and pooled equimolarly.

    Amplification:

    Article Title: Circulating tumor DNA and circulating tumor cells in metastatic triple negative breast cancer patients.
    Article Snippet: .. One μl of the diluted PCR product was subjected to another round of amplification with 0.4 μM indexing primers containing the adapter sequences needed for 454 sequencing (Microsynth), 0.05 U/μl Fast Start High Fidelity polymerase (Roche) and 200 μM dNTPs in 1X High Fidelity Buffer 2 (Roche) in a reaction volume of 15 μl at 95°C for 3 min, 25 cycles of 95°C for 15 s, 60°C for 45 s, and 72°C for 1 min, followed by 72°C for 8 min. .. The PCR products were analyzed on DNA 1000 chips (Agilent) on the Agilent 2100 Bioanalyzer and pooled equimolarly.

    Sequencing:

    Article Title: Circulating tumor DNA and circulating tumor cells in metastatic triple negative breast cancer patients.
    Article Snippet: .. One μl of the diluted PCR product was subjected to another round of amplification with 0.4 μM indexing primers containing the adapter sequences needed for 454 sequencing (Microsynth), 0.05 U/μl Fast Start High Fidelity polymerase (Roche) and 200 μM dNTPs in 1X High Fidelity Buffer 2 (Roche) in a reaction volume of 15 μl at 95°C for 3 min, 25 cycles of 95°C for 15 s, 60°C for 45 s, and 72°C for 1 min, followed by 72°C for 8 min. .. The PCR products were analyzed on DNA 1000 chips (Agilent) on the Agilent 2100 Bioanalyzer and pooled equimolarly.



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    Image Search Results


    We obtained gut microbial DNA from lab-reared stickleback fed chironomids (15 females, 13 males) and Daphnia (14 females, 13 males). We obtained a total of 5,226,827 sequence reads from 15 females (red points) and 13 males (blue points) experimentally fed chironomids (circles), and 14 females and 13 males fed Daphnia (triangles). We averaged 54,446 sequence reads per sample (range: 406–299,947; mean of 31,409), identified as 1,460 microbial OTUs. Here we plot individuals’ scores along the first two linear DFA of microbiota composition (unweighted PCoA axes 1–21) that separate groups (combinations of sex and diet). Larger points with s.e. bars indicate group means. Within each sex, lines represent the diet effect (connecting means for Daphnia - to chironomid-fed fish); the angle between these lines emphasizes the sex-dependent diet effects. In particular, the following taxa are more common in Daphnia -fed males and chironomid-fed females: OTUs in the families Hyphomonadaceae ( P =0.0000014), Isosphaeraceae ( P =0.00006), Haliangiaceae ( P =0.00016), Staphylococcaceae ( P =0.0015), Pirellulaceae ( P =0.0018), Aeromonadaceae ( P =0.0035) and Saprospiraceae ( P =0.0036), and in the genera Burkholderia ( P =0.000010), Veillonella ( P =0.0000012), Staphylococcus ( P =0.000018), Stenotrophomonas ( P =0.00135) and Paracoccus ( P =0.0018).

    Journal: Nature Communications

    Article Title: Individual diet has sex-dependent effects on vertebrate gut microbiota

    doi: 10.1038/ncomms5500

    Figure Lengend Snippet: We obtained gut microbial DNA from lab-reared stickleback fed chironomids (15 females, 13 males) and Daphnia (14 females, 13 males). We obtained a total of 5,226,827 sequence reads from 15 females (red points) and 13 males (blue points) experimentally fed chironomids (circles), and 14 females and 13 males fed Daphnia (triangles). We averaged 54,446 sequence reads per sample (range: 406–299,947; mean of 31,409), identified as 1,460 microbial OTUs. Here we plot individuals’ scores along the first two linear DFA of microbiota composition (unweighted PCoA axes 1–21) that separate groups (combinations of sex and diet). Larger points with s.e. bars indicate group means. Within each sex, lines represent the diet effect (connecting means for Daphnia - to chironomid-fed fish); the angle between these lines emphasizes the sex-dependent diet effects. In particular, the following taxa are more common in Daphnia -fed males and chironomid-fed females: OTUs in the families Hyphomonadaceae ( P =0.0000014), Isosphaeraceae ( P =0.00006), Haliangiaceae ( P =0.00016), Staphylococcaceae ( P =0.0015), Pirellulaceae ( P =0.0018), Aeromonadaceae ( P =0.0035) and Saprospiraceae ( P =0.0036), and in the genera Burkholderia ( P =0.000010), Veillonella ( P =0.0000012), Staphylococcus ( P =0.000018), Stenotrophomonas ( P =0.00135) and Paracoccus ( P =0.0018).

    Article Snippet: The V4 region of the 16S rRNA gene was amplified using bar-coded fusion primers (F515/R806) with the 454 A Titanium sequencing adapter at the EnGenCore Facility at the University of South Carolina.

    Techniques: Sequencing